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  • 董芳,张道微,黄艳岚,周虹,余应弘,张超凡.湖南甘薯种质资源曲叶病毒检测分析初报[J].植物遗传资源学报,2017,18(6):1088-1104.    [点击复制]
  • dongfang,周虹,余应弘,张超凡.Detection and Infection Analysis of Sweet Potato Leaf Curl Virus of Sweet Potato Germplasm Resources in Hunan Province[J].植物遗传资源学报,2017,18(6):1088-1104.   [点击复制]
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湖南甘薯种质资源曲叶病毒检测分析初报
董芳1, 张道微2, 黄艳岚2, 周虹2, 余应弘3, 张超凡2
0
(1.中南大学研究生院隆平分院;2.湖南省作物研究所;3.湖南省农业科学院)
摘要:
研究依托“第三次全国农作物种质资源普查与收集行动”,建立了一套快速有效的甘薯曲叶病毒(SPLCV)巣式PCR(Nested PCR)检测方法,对湖南省收集的246份甘薯资源进行了甘薯曲叶病毒检测,通过甘薯资源曲叶病毒感染率分析研究湖南地区甘薯曲叶病毒的侵染和分布情况。检测结果显示,湖南全省甘薯曲叶病毒的侵染率为8.1%,共有来源于邵阳、长沙、永州和株洲四个地区的14份甘薯资源检出甘薯曲叶病毒,侵染率分别为17.6%、14.5%、7.1%和6.7%,具有较大的地域差异性。该研究首次利用巢式PCR技术对湖南地区甘薯曲叶病毒进行检测和调查,为湖南地区甘薯曲叶病毒侵染情况提供了数据参考,同时也为甘薯资源的保存、繁殖以及后续的开发与利用提供了重要的技术支撑。
关键词:  甘薯曲叶病毒  巣式PCR  甘薯种质资源  病毒检测
DOI:10.13430/j.cnki.jpgr.2017.06.011
投稿时间:2017-01-05修订日期:2017-03-06
基金项目:中南大学中央高校基本科研业务费专项资金(2015zzts326);湖南省自然科学基金(2016JJ6070);第三次全国农作物种质资源普查与收集行动湖南种质资源保护费项目(1120162130135252036)
Detection and Infection Analysis of Sweet Potato Leaf Curl Virus of Sweet Potato Germplasm Resources in Hunan Province
Abstract:
Based on the third national census of crop germplasm resources and collecting action, 246 sweet potato germplasm resources were collected from 14 regions in Hunan Province, and a set of rapid and effective nested PCR detection method for sweet potato leaf curl virus (SPLCV) was established to investigate the situation of infection and distribution of SPLCV in Hunan province through the rate of virus infection. The results showed that the infection rate of SPLCV was 8.1% in Hunan, and there are fourteen sweet potato resources contained sweet potato leaf curl virus in the four regions of Shaoyang, Changsha, Yongzhou and Zhuzhou, and the infection rate of SPLCV was17.6%, 14.5%, 7.1% and 6.7% respectively, which had great regional differences. This study was the first time to detect and investigate SPLCV in Hunan province, which provided data reference for the situation of infection and distribution of SPLCV in Hunan province. It also provided important technical support for the preservation, reproduction and subsequent development and utilization of sweet potato resources.
Key words:  Sweet potato leaf curl virus  Nested PCR  Sweet potato germplasm resources  Virus detection

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