引用本文
  • 马庆华,李京璟,赵天田,梁丽松,王贵禧.基于EST-SSR标记的平欧杂种榛品种鉴定[J].植物遗传资源学报,2017,18(5):952-959.    [点击复制]
  • MA Qing-hua,LI Jing-jing,ZHAO Tian-tian,LIANG Li-song,WANG Gui-xi.Cultivar Identification of Ping’ou Hybrid HazelnutBased on EST-SSR Markers[J].植物遗传资源学报,2017,18(5):952-959.   [点击复制]
【打印本页】 【在线阅读全文】【下载PDF全文】 查看/发表评论下载PDF阅读器关闭

←前一篇|后一篇→

过刊浏览    高级检索

本文已被:浏览 11次   下载  本文二维码信息
码上扫一扫!
基于EST-SSR标记的平欧杂种榛品种鉴定
马庆华,李京璟,赵天田,梁丽松,王贵禧
0
(中国林业科学研究院林业研究所/国家林业局林木培育实验室/林木遗传育种国家重点实验室)
摘要:
为建立准确、高效的平欧杂种榛品种鉴定方法,本研究收集目前国内普遍引种的平欧杂种榛品种(品系)43个,选用课题组已开发的12对EST-SSR引物进行PCR扩增和毛细管电泳分析,进行引物筛选和品种鉴定方法研究。结果表明:不同引物在供试样品中的PIC值为0.3800-0.7839,平均为0.5113,各引物组经毛细管电泳均显示出了良好的扩增效果;平欧杂种榛品种(品系)间的亲缘关系较为复杂,其中薄壳红和平欧62、平欧3和平欧7、平欧30和平欧48的遗传距离最近;引物CAF-2、CAF-3、CAF-12和CAF-13组合使用,可完全区分43个平欧杂种榛品种(品系),其中引物CAF-2与CAF-13组合使用,可鉴定16个主栽品种(品系)。上述研究结果为平欧杂种榛的品种鉴定提供了便捷、有效的方法,可为其他同类研究提供参考。
关键词:  平欧杂种榛  EST-SSR标记  亲缘关系  品种鉴定
DOI:10.13430/j.cnki.jpgr.2017.05.018
投稿时间:2017-01-05修订日期:2017-02-17
基金项目:中国林科院2017年基本科研业务费重点项目子任务“榛子提质增效关键技术研究”(CAFYBB2017ZA004-9)
Cultivar Identification of Ping’ou Hybrid HazelnutBased on EST-SSR Markers
MA Qing-hua,LI Jing-jing,ZHAO Tian-tian,LIANG Li-song,WANG Gui-xi
(Research Institute of Forestry,Chinese Academy of Forestry/Key Laboratory of Forestry Silviculture of State Forestry Administration/State/Key Laboratory of Tree Genetics and Breeding)
Abstract:
The objective of this study is to establish an accurate and high efficiency method for Ping’ou hybrid hazelnut cultivar identification. 43 cultivars/varieties of Ping’ou hybrid hazelnut that were introduced throughout China were used in this experiment. 12 polymorphic EST-SSR markers that developed in the previous study were used to select the high efficient marker combination for cultivar identification. The result showed that: the PIC value of the 12 EST-SSR markers among the 43 samples ranged from 0.3800 to 0.7839, with the average value of 0.5113. All the markers showed good polymorphism and amplification result when visualized by capillary electrophoresis. The genetic relationship of Ping’ou hybrid hazelnut showed very complex due to their breeding method. The genetic distance of Bokehong and Ping’ou 62, Ping’ou 3 and Ping’ou 7, Ping’ou 30 and Ping’ou 48 were the lowest among all the samples. Finally, the combination of four EST-SSR markers, CAF-2, CAF-3, CAF-12 and CAF-13, could identify 43 cultivars/varieties of Ping’ou hybrid hazelnut, the combination of CAF-2 and CAF-13 could identify 16 main cultivars/varieties. The result offered an accurate, convenient and efficiency method for cultivar identification of Ping’ou hybrid hazelnut and could also offer reference for the relative researches.
Key words:  Ping’ou hybrid hazelnut  EST-SSR markers  genetic relationship  cultivar identification

用微信扫一扫

用微信扫一扫