豌豆种质资源芽期耐旱性评价及耐旱种质筛选
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辽宁省科技厅面上项目(原优秀人才培育2015020790);国家现代农业产业技术体系建设专项(CARS-13)


Evaluation and Screening of Pea Germplasm Resources for Drought Resistance at Bud Stage
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    摘要:

    试验采用PEG-6000作为渗透介质,研究了不同浓度PEG-6000旱胁迫下4份豌豆种质的发芽势、发芽率、发芽指数及简易活力指数四个指标的变化动态,确定了豌豆芽期耐旱性鉴定适宜PEG-6000浓度为20%-25%。进而以22%PEG-6000模拟旱胁迫对来自我国18个不同省份的87份豌豆种质资源进行耐旱性鉴定,主成分分析确定了相对发芽势、相对发芽率等7项指标作为耐旱综合评价因子,利用隶属函数法,筛选出1份高抗资源(G0002457,白豌豆),7份抗旱种质。为豌豆耐旱品种改良奠定基础.

    Abstract:

    PEG-6000 was used as permeability media, to study dynamic change of germination energy, germination rate, germination index and vigor index of four pea varieties under six different PEG-6000 concentrations. The results showed that 20%-25% of PEG-6000 was the suitable concentration range to screen drought resistance resources at bud stage for pea. Drought resistance of 87 pea accessions from 18 different provinces of China were evaluated with 22% PEG-6000 solution. The principal component analysis results showed that relative values of germination energy, germination rate, radical length, radical fresh biomass, and germination drought resistance index, germination stress index, vigor index were the main impact factors to evaluate drought resistance for pea. Based on the subordinative function analysis of screening results, one germplasm (G0002457) with the highest level of drought resistance and seven general drought resistant germplasms were identified and recommended to be used in the drought tolerance improvement for pea breeding.

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李玲,沈宝宇,张天静,等.豌豆种质资源芽期耐旱性评价及耐旱种质筛选[J].植物遗传资源学报,2017,18(4):778-785.

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  • 收稿日期:2016-12-10
  • 最后修改日期:2017-01-12
  • 录用日期:2017-02-21
  • 在线发布日期: 2017-07-14
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