斑茅割手密复合体(GXAS07-6-1)及其与甘蔗F1 的GISH 分析
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广西农业科学院基本科研业务专项(2015YT04);广西“八桂学者”专项经费


GISH Analysis of Intergeneric Complex ( Erianthus arundinaceus ?Saccharum spontaneum) and its F1 Hybrids from crosses with Sugarcanes ( Saccharum spp.)
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    摘要:

    斑茅割手密复合体(GXAS07-6-1)是广西斑茅和广西割手密的属间杂种,聚集了双亲的优点。本研究利用Alu-like方法对3份甘蔗与GXAS07-6-1F1材料进行真实性鉴定,基于基因组原位杂交技术( GISH)对其及其父本GXAS07-6-1染色体组成及核型进行分析。研究结果表明:3份F1材料为GXAS07-6-1的真杂种;GXAS07-6-1的染色体众数为62条,其中30条来自斑茅,32条来自割手密,核型分类属于1B,其染色体按“n n”方式传递;GXASF108-2-17、GXASF108-2-22、GXASF108-2-32的染色体数目为78~80条,其中69~71条来自甘蔗及广西割手密,9~11条来自斑茅,3份F1的核型分类分别属于2B、1B、1B,染色体方式传递均为“n n”。GXAS07-6-1及3 份F1材料中都未发现有染色体的交换或易位现象。甘蔗与斑割复合体杂交,斑茅染色体在亲子间传递过程存在丢失现象。

    Abstract:

    GXAS07-6-1, an intergeneric hybrid of E. arundinaceus and S. spontaneum L. has the advantages of its parents, which make it an important material for sugarcane innovation breeding.In this study, The F1 hybrids GXAS08-2-17, GXAS08-2-22 and GXAS08-2-32 were identified as a true progenies of E.arundinaceus by the method of Alu-like. And the chromosomes composition of the GXAS07-6-1 and the F1 generation were analyzed using the genomic in situ hybridization (GISH) technique. The results showed that there were 62 chromosomes in GXAS07-6-1,of which 30 are from E. arundinaceus and 32 are from S.spontaneum. The somatic chromosome karyotypic types of GXAS07-6-1 is 1B , following n n transmission. Furthermore, there were 75 to 84 chromosomes in the F1 hybrids, of which 78 to 80 are from Saccharum spp and S. spontaneum GXS79-9, and 9 to 11 are from E. arundinaceus. The somatic chromosome karyotypic types of 3 F1 progeny were 2B, 1B, 1B, respectively, following n n transmission. Chromosome translocation and exchange were not observed in the intergeneric hybrid GXAS07-6-1 and it’s F1 hybrids as well . We also confirmed the occurrence of chromosome missing from E. arundinaceus.in the F1 progeny.

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黄玉新,罗霆,林秀琴,等.斑茅割手密复合体(GXAS07-6-1)及其与甘蔗F1 的GISH 分析[J].植物遗传资源学报,2017,18(3):461-466.

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  • 收稿日期:2016-09-19
  • 最后修改日期:2016-10-26
  • 录用日期:2016-12-21
  • 在线发布日期: 2017-05-19
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