基于SSR标记的陆地棉早熟相关种质遗传多样性分析
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国家棉花产业技术体系(CARS-18-03)


Genetic Diversity Analysis of Early-maturity Related Germplasm in Upland Cotton Based on SSR Markers
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    摘要:

    丰富的遗传变异对于提高作物的环境适应性和遗传改良进度至关重要。为了解我国早熟陆地棉种质资源遗传多样性,本研究利用136对SSR引物对186份陆地棉材料(96份早熟陆地棉材料和90份晚熟陆地棉材料)进行了遗传多样性分析,共检测到等位基因变异355个,平均2.61个。在早熟棉材料中,有134对多态性SSR引物,扩增出341个条带,平均2.54个;晚熟材料中有133对多态性SSR引物,扩增出345个条带,平均2.59个。早熟棉材料的位点多态信息含量(PIC)、有效等位基因数(Ne)、基因型多样性(H′)分别为0.684、3.994和1.361;晚熟棉材料的PIC、Ne、H′分别为0.668、3.852和1.343。早熟棉材料和晚熟棉材料的Jaccard相似性系数分别在0.349-0.935和0.270-0.907之间, 平均为0.635、0.666 。遗传相似性系数总体平均值接近, 但早熟棉变化范围较晚熟棉小。用类平均法(UPGMA)进行聚类可将186份材料分成2个类群。总体上来看,供试材料遗传相似性系数较高,说明我国陆地棉早熟相关种质遗传基础狭窄。本研究结果为早熟棉育种亲本选配,早熟棉种质创新提供依据。

    Abstract:

    Diverse genetic variation is of great importance for crop ecological adaption and genetic improvement.In order to know the genetic diversity of early maturity in upland cotton, 186 upland cotton (96 early-maturity and 90 later-maturity upland cotton) were explored using136 simple sequence repeat (SSR) markers, and a total of 355 distinctive loci were detected with an average of 2.61. A total of 341 distinctive loci were amplified from 134 SSRs with an average of 2.54 per SSR in early-maturity upland cotton and 345 distinctive loci were amplified from 133 SSRs with an average of 2.59 per SSR in later-maturity upland cotton. Mean polymorphism information content (PIC), average effective numbers of alleles (Ne) and average genotype diversity index (H′) were 0.684, 3.994 and 1.361 in early-maturity upland cotton, and 0.668, 3.852 and 1.343 in later-maturity upland cotton, respectively. The similarity coefficients were similar between early-maturity upland cotton and later-maturity upland cotton, ranging from 0.349 to 0.935 in early-maturity upland cotton with an average of 0.635, and ranged from 0.270 to 0.907 in later-maturity upland cotton with an average of 0.666, respectively. These varieties were clustered into two groups using unweighted pair group method analysis (UPGMA). The high genetic similarity coefficients of the early-maturity upland cotton indicated the genetic background of early-maturity upland cotton was narrow in China. These results are useful for assessing cotton pedigrees, improving cotton hybrids, and ultimately allowing for the improved utilization of early-maturity cotton.

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赵树琪,梁冰,李黎贝,等.基于SSR标记的陆地棉早熟相关种质遗传多样性分析[J].植物遗传资源学报,2016,17(4):599-606.

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  • 收稿日期:2015-09-18
  • 最后修改日期:2015-10-19
  • 录用日期:2016-01-29
  • 在线发布日期: 2016-07-07
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