The investigation of twenty one counties in Guizhou province were carried out by the project of “investigation of biological resources of agriculture in Guizhou province”during 2012-1014. The results showed that of all twenty one counties systematically investigated have minority people to live , and all different minority people has their different traditional culture and customs of life， which endowed the local crop germplasm resources with rich culture connotation in the passed from generation to generation. Guizhou province is in the Yunnan-Guizhou plateau where the landform is complexity and the climate is diverse. The diversity of natural condition and traditional culture in the minority areas resulted in rich resources of the crop rare germplasm and wild relatives. The rare germplasm resources of crop and wild relatives will be some utilization value in crop classification, new variety breeding and commercial development.