The study of MybA genotypes in different grape cultivars can provide the basis for identification and classification of grape cultivars as well as selection of parents in grape breeding. The result of MybA genotyping in 118 primary core collection of grape genetic resources shows that most Vitis vinifera cultivars and their hybrids have VvmybA1a which is an allele of VvmybA1 gene, with the exception of 10 Vitis vinifera cultivars and their hybrids. VvmybA1, VvmybA2 and VvmybA3 genes were all detected in most Vitis vinifera, hybrids of V. labrusca and V. Vinifera, and hybrid wine grapes, except for a few grape cultivars with VvmybA2 or VvmybA3 undetected. Hybrids of V. amurensis and V. vinifera, Beimei, Xiongyuebai and Gongniangyihao have all VvmybA1, VvmybA2 and VvmybA3, while Beichun and Beihong have only VvmybA1 and VvmybA3. VlmybA2 gene were only detected in some hybrids of V. labrusca and V. vinifera, and hybrid wine grapes. It is noteworthy that in five Vitis labrusca cultivars VvmybA1a (typical genotype of V. vinifera) but not VlmybA2 was detected. We speculate that they are the hybrids of V. labrusca and V. vinifera, with more V. labrusca parentage, and they are not the pure Vitis labrusca.