云南地方稻waxy基因序列多样性分析
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科技部基础性工作专项“贵州农业生物资源调查”(2012FY110200);国家自然科学基金“抗稻瘟病基因Pid2进化与专化抗性分子机制研究”(31360331)


The Diversity Analysis of the waxy gene of the Local Rice Variety from Yunnan
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    摘要:

    本文利用特异引物Wx-F/AG-2对来源云南16个州市64个县的252份地方稻种waxy基因中含有微卫星序列(CT)n和第一内含子的序列进行PCR扩增并测序。结果表明在260个碱基的序列内检测到4个变异位点:第一内含子上游56位处(CT)n存在重复数10,11,12,14,16,17,18,19,20,21的变异;第一内含子 1位存在G/T变异,而T只出现在CT重复大于16的品种中; 86-88存在ATA/GTA/A-- 三种变异; 128位处(AATT)n存在 5和6两种重复数变异。根据这4个变异位点,可将252个云南地方稻种归为16种基因型,其中Wx4(32.54%),Wx9(13.89%),Wx12(12.7%),Wx5(12.3% ),Wx1(8.33%),Wx11(7.94%)是主要类型,其他类型频率较低。籼/粳亚种、水/陆稻和粘/糯中存在基因型种类和基因型频率两方面的差异,籼稻/粳稻、水/陆稻和粘/糯稻各亚种或生态型均有独享的基因型,共享基因型频率也存在差异,表明亚种间或生态型间发生了一定的遗传分化。基因型地理分布表明,临沧、普洱基因型种类最丰富,以之为中心向外扩展,基因型种类有减少的趋势,第一内含子 1位的T主要分布在临沧、普洱、版纳、德宏等南部地区。本研究揭示了云南地方稻种群体waxy基因的变异和分布特点。

    Abstract:

    The waxy gene sequences including microsatellite sequence (CT)n and the part of first intron were amplificated and sequenced using the PCR pecific primers Wx-F / AG-2 for 252 rice landraces from 16 areas 64 counties of Yunnan. The results showed that Four variable sites were detected in the waxy gene sequences incuding 260 bases. The (CT)n variation duplicate number 10, 11, 12, 14, 16, 17, 18, 19, 20, 21 was tested at site 56 of the first intron upstream; The variation G / T was present in the first intron 1 site and T only was present in the cultivars with (CT)n length greater than 16 breeds; There were three variations include ATA / GTA /A at 86-88 site; There were two (AATT) n variations (5 and 6) duplicate number at 128 site. According to these four polymorphic sites, the 252 Yunnan rice landraces were classified into 16 genotypes, which Wx4 (32.54%), Wx9 (13.89%), Wx12 (12.7%), Wx5 (12.3%), Wx1 (8.33%), Wx11 (7.94%) were the main type, other types of frequency were lower. There were differentiation in genotype types and genotype frequencies between Indica / japonica subspecies, water / upland rice and sticky / glutinous rice. The indica / japonica, water / upland rice and no-glutinous / glutinous rice subspecies or ecotypes had exclusive genotype, shared genotype frequency also had differences. This indicated that genetic differentiation were occurred between subspecies or ecotypes. Geographical distribution of genotypes showed that Lincang, Puer genotype species were the most abundant, and expansion outward as it the center, genotype species were decreasing trend, the T at the first intron 1 site were mainly distributed in Lincang, Pu'er, Banna, Dehong and other southern regions. This study revealed the waxy gene the variability and distribution of Yunnan rice landraces groups.

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马继琼,孙一丁,杨奕,等.云南地方稻waxy基因序列多样性分析[J].植物遗传资源学报,2016,17(2):365-370.

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  • 收稿日期:2015-03-18
  • 最后修改日期:2016-01-06
  • 录用日期:2016-01-20
  • 在线发布日期: 2016-03-15
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