野生扁蓿豆种质资源AFLP遗传多样性的分析
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“作物种质资源收集、编目、入库与安全保存”(2013BAD01B01);农作物种质资源保护与利用项目“牧草种质资源收集、编目、更新与利用”(2015NWB037)


Genetic diversity in Medicago ruthenica (L.) Trautv. populations using AFLP
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This study was supported by the National Key Technology R&D Program (No. 2013BAD01B01); Ministry of Agriculture, Crop Germplasm Resources Protection and Utilization Project (2014NWB037)

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    摘要:

    阐明不同分布区域的野生扁蓿豆遗传多样性。用AFLP标记对来自中国河北省、辽宁省、吉林省、山西省、内蒙古自治区和西藏自治区的10份野生扁蓿豆居群进行遗传多样性分析。从64对AFLP引物组合中,筛选出8对扩增条带清晰、多态性高的引物组合,8对引物共扩增出640个位点,其中多态性位点有472个,多态位点百分率为73.8%;Nei’s基因多样性指数平均为0.157,Shannon 多态性信息指数平均为0.099;居群间遗传相似系数(GS) 范围平均值为0.827。聚类和主成分分析可将10个扁蓿豆居群聚为3大类,结果与居群的地理分布大致相符,呈一定的地域性分布规律。由此可见,AFLP分子标记研究结果能较好地揭示扁蓿豆居群间的遗传多样性。对来自中国各地的野生扁蓿豆资源的广泛收集、评价和基因资源的保护有重要的意义。

    Abstract:

    The genetic relationship of 10 M. ruthenica populations from Hebei, Liaoning, Jilin, Shanxi Provinces and Inner Mongolia and Tibet autonomous regions of China were analysis by AFLP. 8 primers with strong polymorphism and good repeatability were screened from 64 AFLP primers, generating a total of 640 bands of which 472 (73.8%) showed polymorphism. On average, the Nei’s gene diversity, Shannon’s information index and genetic similarity index (GS) were 0.157, 0.099 and 0.827, respectively. 10 M. ruthenica germplasms were divided into 3 groups with the higher correlation of geography resource by the cluster analysis and PC results. AFLP is the candidate molecular marker to reveal the genetic diversity of M. ruthenica. The results indicated that the wild population of M. ruthenica from the china had a rich genetic diversity in AFLP genotype and thus it was of great important to explore genetic evolution and gene resources and to preserve the local M. ruthenica populations in this area and expanded area.

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引用本文

李鸿雁,李志勇,辛霞,等.野生扁蓿豆种质资源AFLP遗传多样性的分析[J].植物遗传资源学报,2016,17(1):78-83.

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  • 收稿日期:2015-02-02
  • 最后修改日期:2015-03-25
  • 录用日期:2015-12-07
  • 在线发布日期: 2016-01-19
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