小麦“BS20×Fu3”DH群体SSR遗传图谱的构建及不育基因的QTL定位
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

国家自然科学基金项目(30840050)、国家高技术研究与发展计划(863)计划项目(2009AA101102; 2006AA100102)


Construction of an SSR genetic linkage map of wheat using DH population derived from BS20×Fu3 and mapping QTLs for sterile gene
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    以小麦光温敏核雄性不育系BS20×Fu3 双单倍体(DH)群体的289个系为材料,从1112对SSR和EST-SSR引物中筛选出多态性的引物243对,利用其中128个SSR和6个EST-SSR标记构建遗传连锁图谱,该图谱覆盖长度为2749.2cM,分布在小麦的19个连锁群(除4D、6A),不同连锁群标记数为2-15 个,长度在15.3-244.4cM 之间,平均长度为144.7cM,标记之间平均遗传距离为17.4cM。同时构建三个DNA池(包括恢复池、北京不育池和阜阳不育池),用分离群体分组分析法(BSA)对育性进行分析,筛出的多态性引物为Wmc264、Wmc73、Xgwm350,分布在3A、5B、2A/7D染色体上。同时用混合线性复合区间作图法(MCIM)对育性进行QTL分析,当F>7.5时,检测到6个主效QTL,用复合区间作图法(CIM)对育性进行QTL分析,当LOD值>2.5时,共检测到13个主效QTL,两种方法检测到一致的QTL有3个,分别为1BL的Wmc365-cfa2129、2BS的Wmc602-Xgwm148和3AL的Wmc264a-cfa2262区间的QTL。综合BSA和QTL的结果,位于1BL、2BS、和3AL上的小麦光温敏不育基因是真实的。

    Abstract:

    The genetic linkage map of wheat was constructed with DH population consisting of 289 individuals from a cross between BS20 and Fu3. 1112 SSR and EST-SSR primers were used to detect the polymorphisms between parents. Applying QGA_CN software to carry out linkage analysis ,the genetic map contains 19 chromosomes (without 4D and 6A) which included 128 SSR and 6 EST-SSR markers. The linkage map covered 2749.2 cM and the average distance was 17.4 cM. All 19 linkage groups consisted of 2-15 markers,the coverage of length from 15.3 to 244.4 cM. At the same time,we constructed three DNA pools including Beijing sterile pool,Fuyang sterile pool and restorer pool for screening the sterile genes using BSA method,and these primers were Wmc264, Wmc73 and Xgwm350,which distributed by chromosomes 3A, 5B and 2A/7D. Six major QTLs were detected when the F value>7.5 by mixed-model composite interval mapping.Thirteen major QTLs were found when the LOD value > 2.5 with composite interval mapping.Three QTLs were shared from two methods, the sterile genes were located on chromosomes 1BL , 2BS and 3AL, the marker intervals were Wmc365-cfa2129,Wmc602-Xgwm148 and Wmc264a-cfa2262 respectively,after the analysis of BSA and QTL.

    参考文献
    相似文献
    引证文献
引用本文

田再民,张立平,王丽辉,等.小麦“BS20×Fu3”DH群体SSR遗传图谱的构建及不育基因的QTL定位[J].植物遗传资源学报,2015,16(4):857-867.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2014-10-08
  • 最后修改日期:2015-01-05
  • 录用日期:2015-05-27
  • 在线发布日期: 2015-06-11
  • 出版日期:
您是第位访问者
ICP:京ICP备09069690号-23
京ICP备09069690号-23
植物遗传资源学报 ® 2024 版权所有
技术支持:北京勤云科技发展有限公司