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  • 张凤洁,李雪垠,高世庆,唐益苗,王爱萍.普通小麦祖先种类TaNAC2a基因的生物信息和表达分析[J].植物遗传资源学报,2014,15(5):1012-1022.    [点击复制]
  • zhangfengjie,李雪垠,高世庆,唐益苗,王爱萍.Bioinformatic analysis and expression pattern of NAC transcription factor genes homologous to TaNAC2a in bread wheat progenitors[J].植物遗传资源学报,2014,15(5):1012-1022.   [点击复制]
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普通小麦祖先种类TaNAC2a基因的生物信息和表达分析
张凤洁,李雪垠,高世庆,唐益苗,王爱萍
0
(山西农业大学农学院;西北农林科技大学农学院;北京杂交小麦工程技术研究中心)
摘要:
本研究采用生物信息学方法,利用核酸、蛋白数据库对普通小麦祖先种乌拉尔图小麦(Triticum urartu L.)和粗山羊草(Aegilops tauschii L.)NAC 转录因子基因家族进行分析,分别鉴定出 107、126 个 NAC 蛋白家族成员,根据拟南芥、水稻 NAC 基因家族分类系统,将其分为15 个亚族。通过与抗逆相关基因 TaNAC2a 进行同源进化树分析,发现 5 个 TuNAC、6 个 AetNAC 基因与其高度同源,对这些基因的蛋白结构域、基因结构、启动子顺式作用元件及组织表达特性进行分析。结果表明:11 个 NAC 蛋白具有典型的 NAC 结构域。进化关系较近的基因具有相似基因结构;启动子区域预测发现其均含有逆境胁迫响应作用元件。实时荧光定量 PCR 结果显示,TuNAC、AetNAC 基因分别在乌拉尔图小麦和粗山羊草根、胚芽鞘、叶组织中均有表达,并呈现出明显的组织表达特异性。通过芯片表达数据和逆境胁迫基因表达试验推测 AetNAC2c 基因可能参与植物干旱胁迫响应,AetNAC2b 可能参与调控植物的耐旱、耐低温胁迫反应。上述分析结果为普通小麦祖先种基因家族的系统研究,优异候选功能基因的预测、筛选提供了实验依据。
关键词:  乌拉尔图小麦  粗山羊草  NAC  生物信息学  表达特性
DOI:
投稿时间:2014-04-28修订日期:2014-05-29
基金项目:
Bioinformatic analysis and expression pattern of NAC transcription factor genes homologous to TaNAC2a in bread wheat progenitors
zhangfengjie,李雪垠,高世庆,唐益苗,王爱萍
()
Abstract:
In this study,107 and 126 NAC transcription factor family members in Triticum urartu L. and Aegilops tauschii L. genome were identified respectively by hmm profile search..They were further classified into two groups, consisting of 15 subgroups based on the classification method of Arabidopsis and rice. Phylogenetic analysis revealed that 5 TuNAC genes and 6 AetNAC genes were found to be similar in protein sequence with TaNAC2a, which had been reported involving in the response to diverse stresses in plant. Phyletic evolution, protein domains, gene structure, cis-acting elements in the promoter region and tissue-specific expression pattern of these 11 NAC genes were further analyzed. Results showed that these 11 NAC proteins possessed typical NAC domains and several pairs of genes with close evolutionary relationship had similar gene structure. Prediction of cis-acting elements revealed that they all had stress-responsive cis-acting elements in their promoter region. Gene expression pattern analysis revealed that the transcripts of TuNAC, AetNAC genes were detected in root, coleoptile and leaf of Triticum urartu and Aegilops tauschii, respectively, and exhibited obvious tissue-specific expression pattern. It was speculated from microarray data and expression patten under stress conditions that AetNAC2c had a role in the response to drought stress and AetNAC2b was more likely to be involved in the regulation of plant reaction to low temperature and drought stress. Our analysis lays a foundation for systematical research of gene family in bread wheat progenitors and provides an experimental basis for the identification of excellent functional genes.
Key words:  Triticum urartu  Aegilops tauschii  NAC  bioinformatics  expression charactertics

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