黑龙江省野生秋子梨群体遗传结构的荧光AFLP分析
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国家自然科学基金项目(31171932);国家重点基础研究发展计划课题(2011CB100606)


Genetic Structure Analyses Based on Fluorescent-AFLP Markers of Wild Populations of Pyrus ussuriensis Maxim in Heilongjiang Provinc
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The National Basic Research Program of China (973 Program);The National Natural Science Foundation of China (General Program, Key Program, Major Research Plan)

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    摘要:

    摘 要:以来自黑龙江省海林市、东宁县和孙吴县3个居群的90个野生秋子梨株系为试材,利用荧光AFLP标记技术对野生秋子梨群体遗传多样性及遗传结构进行了分析,旨在为野生秋子梨种质资源的保护和利用提供科学依据。结果表明:野生秋子梨3个居群种级水平多态位点百分比(P)为82.95%,Nei基因多样度(H)为0.1467,香农信息指数(I)为0.2397,野生秋子梨遗传多样性水平较低;3个居群的多态位点百分比、基因多样度及香农信息指数比较东宁居群>海林居群>孙吴居群,居群水平上东宁居群的遗传多样性较丰富;基因分化系数(GST =0.1008)显示,野生秋子梨的遗传变异主要发生在于居群内;野生秋子梨居群间存在较大的基因流(Nm =4.4603),说明3个野生秋子梨居群间存在着较频繁的基因交流;UPGMA聚类分析结果表明,3个居群是相对独立的孟德尔群体,且海林居群和东宁居群的遗传关系较近。3个居群中东宁居群遗传多样性最丰富,是进行原生境保护优先考虑的居群。

    Abstract:

    Abstract:Employing fluorescent AFLP marker, we analyzed population genetic diversity and genetic structure of wild Pyrus ussuriensis with the aim to provide scientific foundation for wild Pyrus ussuriensis germplasm conservation and utilization. 90 accessions from Hailin, Dongning and Sunwu country of Heilongjiang province were selected as the research material. The results showed that the percentage of polymorphic loci (P) was 82.95% at species level, Nei's's gene diversity (H) was 0.1467 and Shannon information index (I) was 0.2397. And the genetic diversity of wild Pyrus ussuriensis Maxim was low. The polymorphic loci (P), Nei’s gene diversity (H) and Shannon information index (I) at population level were sequenced from high to low as Dongning population>Hailin popultion>Sunwu population, which means that genetic diversity of Dongning population is the richest in the three populations. The genetic differentiation coefficient (GST =0.1008) of the three populations showed that genetic variation mainly occurred within populations. There was higher level of gene flow between three populations (Nm=4.4603) which indicated that there were frequent gene exchange between populations. The UPGMA cluster analysis indicated that three populations are mendelian relative independent population, and the closer genetic relationship exist between Hailin and Dongning population. Dongning population has the most abundant genetic diversity and is the preferential population for the implementation of situ germplasm conservation strategies.

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安萌萌,刘畅,何天明,等.黑龙江省野生秋子梨群体遗传结构的荧光AFLP分析[J].植物遗传资源学报,2014,15(4):728-733.

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  • 收稿日期:2013-11-07
  • 最后修改日期:2013-11-17
  • 录用日期:2014-06-09
  • 在线发布日期: 2014-06-10
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