RVA was often used to identify germplasm resources and eating quality of the selected strains, but the RVA pasting temperature(PT) couldn’t accurately reflect gelatinization temperature(GT). Calibrated pasting temperature(PTm) could accurately reflect GT, however, the calibration range of PT, the accuracy rate of using PTm to reflect GT and the influence of SSIIa gene on them weren’t reported in papers. In this study, we use fifty-eight individual plants came from B1F8 breeding line as our materials which had small differences in genetic background, but large differences in gelatinization temperature of their parents. By detecting the genotype(haplotype) of SSIIa gene of the materials, we divided materials into three categories and compared PT、PTm and GT under the genetic background of three different genotype of SSIIa respectively. The main results were as follows: Calibration ranges of PT were apparently different due to the differences existing in genotype of SSIIa, and the average of PT-PTm had very significant difference. The accuracy rate reached 39.66% when we used PT to evaluate GT, while reached 89.66% when we used PTm to evaluate GT. Then using PT and PTm to represent GT respectively, we analyze the relationship between AC and GT of rice, the result showed that AC had a significant negative correlation with GT, which was different with some scholars’ conclusion.