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  • 舒金帅,刘玉梅,方智远,李占省,孙培田,杨丽梅,张黎黎,张扬勇,庄木.青花菜两类雄性不育系花器官形态结构的比较[J].植物遗传资源学报,2014,15(1):113-119.    [点击复制]
  • shu jin shuai,Liu Yumei,方智远,李占省,孙培田,杨丽梅,张黎黎,张扬勇,庄木.Study on the Floral characteristics Characteristics and Sstructure in Two Types of Male Sterile Lines of Bbroccoli(Brassica oleracea var.italica)[J].植物遗传资源学报,2014,15(1):113-119.   [点击复制]
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青花菜两类雄性不育系花器官形态结构的比较
舒金帅,刘玉梅,方智远,李占省,孙培田,杨丽梅,张黎黎,张扬勇,庄木
0
(中国农业科学院蔬菜花卉研究所)
摘要:
青花菜是一种重要的十字花科蔬菜,具有良好的抗癌功效,随着青花菜栽培面积的不断扩大和抗癌药物的开发,对青花菜种子的需求量也不断增加。利用雄性不育系生产青花菜F1代杂交种子是其杂种优势利用的重要途径,但常用的细胞质雄性不育系在生产中存在前期死蕾较重、花蜜量少、种子产量不高等问题,本课题组用优良的青花菜自交系为父本,以甘蓝显性细胞核雄性不育材料79-399-3和细胞质不育材料OguraCMSR3 629为不育源,通过回交转育的方法获得了多个青花菜显性细胞核雄性不育系和细胞质不育系,为了进一步有效利用其青花菜雄性不育系判断其优劣,寻求更好的不育源,本研究以青花菜高代自交系(保持系)8554、8590、93219及由其经多代回交转育而成的两类雄性不育系为试材,对其死蕾数、花器官形态、蜜蜂访花情况进行了比较研究。结果表明,在花蕾长、花蕾直径、单枝死蕾数、花冠直径、雄蕊长、花瓣长、花药长、蜜蜂访花次数、来访蜜蜂数及访花时间等方面,经相同自交系转育而成的显性细胞核雄性不育系(DGMS)与细胞质雄性不育系(CMS)间的存在显著差异差异有统计学意义,总体表现为DGMS明显优于CMS。
关键词:  青花菜  DGMS  CMS  花器官  访花
DOI:
投稿时间:2013-04-22修订日期:2013-05-20
基金项目:现代农业产业技术体系建设专项资金(国家大宗蔬菜产业技术体系);国家科技支撑计划课题(2013BAD01B04);农业部园艺作物生物学与种质创制重点实验室项目。
Study on the Floral characteristics Characteristics and Sstructure in Two Types of Male Sterile Lines of Bbroccoli(Brassica oleracea var.italica)
shu jin shuai,Liu Yumei,方智远,李占省,孙培田,杨丽梅,张黎黎,张扬勇,庄木
()
Abstract:
Broccoli is an important cruciferous vegetable, it has a good anti-cancer effect, with the expanding cultivation area of broccoli and the developing of anticancer drug, the demand for broccoli seeds are increasing. Using male sterile line to produce F1 generation seeds is an important way to utilize of heterosis, but the cytoplasmic male sterile lines commonly used in production have the problems of the buds death are heavier in the early, nectar volume is less and seeds production are low. Our group makes excellent broccoli inbred lines as male parent. cabbage dominant genic male sterility material 79-399-3 and cytoplasmic male sterile material OguraCMSR3 629 as the infertility sources, we have obtained multiple dominant genic male sterile lines and cytoplasmic male sterile lines of broccoli through backcrossing method, in order to further effectively use of broccoli male sterile lines and search for better infertility sources, The the differences of the bud death numbers, floral organ structure and number of or frequecing bees visiting flowers among dominant genic male sterile(DGMS)lines,cytoplasmic male sterile(CMS)lines and inbred lines of broccoli 8554, 8590 and 93219 were studied. The results showed that the differences of the bud length, bud diameter, death buds of single branch, corolla diameter, stamen length, anther length, petal length, the times of visiting bees, the number of visiting bees and the time of bees staying on the flower between DGMS and CMS of lines 8554, 8590 and 93219 were significant. On As athe whole, above characteristics in DGMS lines were significantly better than CMS lines.
Key words:  DGMS  CMS  floral organ  numbers of bees visiting flowers

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