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  • 马婧,刘群,李名扬,王晓斌,眭顺照.2个蜡梅nsLTP基因启动子的克隆及其在烟草中的瞬时表达分析[J].植物遗传资源学报,2012,13(4):601-608.    [点击复制]
  • majing,刘群,limingyang,王晓斌,眭顺照.Cloning and Transient Expression Assay of Two nsLTP Gene Promoters from Chimonanthus Praecox in tobacco[J].植物遗传资源学报,2012,13(4):601-608.   [点击复制]
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2个蜡梅nsLTP基因启动子的克隆及其在烟草中的瞬时表达分析
马婧,刘群,李名扬,王晓斌,眭顺照
0
(西南大学园艺园林学院,重庆市花卉工程技术研究中心,南方山地园艺学教育部重点实验室)
摘要:
利用hiTAIL-PCR法得到了2个蜡梅(Chimonanthus praecox)非特异性脂转移蛋白(non-specific lipid transfer protein)基因家族成员CpLTP3和CpLTP4翻译起始位点上游启动子序列,长度分别为1298bp和838bp。生物信息学分析表明2个序列均存在启动子的基本元件TATA-box和CAAT-box及多个与植物非生物胁迫相关的响应元件。在烟草叶片中的瞬时表达结果表明这两个启动子序列均具备驱动报告基因GUS表达的功能。
关键词:  蜡梅  非特异性脂转移蛋白  启动子  hiTAIL-PCR  胁迫
DOI:
投稿时间:2011-09-23修订日期:2012-05-31
基金项目:国家自然科学基金项目(面上项目,重点项目,重大项目);西南大学研究生科技创新基金优博项目
Cloning and Transient Expression Assay of Two nsLTP Gene Promoters from Chimonanthus Praecox in tobacco
majing,刘群,limingyang,王晓斌,眭顺照
(College of Horticulture and Landscape, Southwest University, Chongqing Engineering Research Center for Floriculture,Key Laboratory of Horticulture Science for Southern Mountainous Regions,Ministry of Education)
Abstract:
The promoter fragments of 1298bp and 838bp upstream from the 5’ upper of Chimonanthus praecox non-specific lipid transfer protein(nsLTP) gene family member CpLTP3 and CpLTP4 were isolated from the genomic DNA of Chimonanthus praecox by hiTAIL-PCR technique respectively. Bioinformatics analysis revealed that the two sequences contained basic cis-elements,such as TATA-box and CAAT-box and many elements involved in the plant abiotic stress. The result of transient expression indicated that all the sequences had the function to drive reporter gene GUS in tobacco.
Key words:  Chimonanthus Praecox  non-specific lipid transfer protein  promoter  hiTAIL-PCR  stress

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