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  • 王荣升,杨庆文.基于叶绿体基因多样性的中国水稻起源进化研究[J].植物遗传资源学报,2011,12(5):686-693.    [点击复制]
  • wangrongsheng.Study on the Origin and Evolution of Cultivated Rice in China Based on Gene Diversity of Chloroplast Genome[J].植物遗传资源学报,2011,12(5):686-693.   [点击复制]
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基于叶绿体基因多样性的中国水稻起源进化研究
王荣升,杨庆文
0
(中国农业科学院作物科学研究所)
摘要:
针对目前亚洲栽培稻起源地和进化途径学说众多、分歧巨大的现状,本研究选择原产中国的98份亚洲栽培稻和125份普通野生稻为材料,对叶绿体中atpA序列、rps16内含子序列、trnP-rpl33间隔区、trnG-trnfM序列、trnT-trnL间隔区序列的五段高突变序列进行测序,利用生物信息学方法进行比对分析,绘制Network网络图,构建系统发育树。结果表明,普通野生稻的Indel和SNP数目均比亚洲栽培稻多,序列多样性丰富;基于单倍型的Network网络图和系统发育树可将所有参试材料归为3个类群,类群I主要为粳稻与普通野生稻,类群II主要为籼稻,类群III主要为普通野生稻,而类群II和类群III亲缘关系较近,提示粳、籼两个亚种可能由偏粳、偏籼的普通野生稻分别进化而来,支持二次起源学说;所有与亚洲栽培稻亲缘关系较近的普通野生稻均来源于华南地区,支持华南地区为我国亚洲栽培稻起源中心的论点。
关键词:  水稻  叶绿体  基因多样性  起源  籼粳分化
DOI:
投稿时间:2011-05-03修订日期:2011-05-28
基金项目:农业部财政专项“农业野生植物保护与可持续利用”;转基因重大专项“转基因水稻环境安全评价”(2008ZX08011-001)
Study on the Origin and Evolution of Cultivated Rice in China Based on Gene Diversity of Chloroplast Genome
wangrongsheng
(China academy of agriculture sciences)
Abstract:
To study the origin and evolution of Asian cultivated rice (Oryza sativa L.), 98 accessions of Asian cultivated rice and 125 accessions of common wild rice (Oryza rufipogon) were selected for sequencing analysis. Through sequencing the atpA gene, rps16 intron, trnP-rpl33 inter spacer, trnG-trnfM inter spacer and trnT-trnL inter spacer in chloroplast genome in rice, the haplotype network and phylogenetic tree were made with bio-information software. The results showed that the tested common wild rice had more Indels and SNPs than the Asian cultivated rice. Both the haplotype network and phylogenetic tree divided all test materials into three groups. GroupⅠconsisted by accessions from the Japonica subspecies and the common wild rice, Group II included mainly the accessions of Indica subspecies, while Group III were of the accessions of the common wild rice. Importantly, Group II and Group III had a close relationship, which indicated that the Japonica and Indica Subspecies were originated from Japonica-like and Indica-like common wild rice separately, supporting the Double Domestication Hypotheses of Asia cultivated rice. All the materials of common wild rice with a close relationship to Asian cultivated rice were identified to be collected from South China, implicating South China might be the origin center of Asian cultivated rice.
Key words:  Rice  Chloroplast  Gene Diversity  Origin  Indica-japonica differentiation

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