180份玉米自交系资源亲缘关系的分子评价
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作物种质资源保护项目(NB08-2130135-(25-31)-11)


Molecular Evaluation of 180 Maize Inbred Lines by SSR Markers
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    摘要:

    利用覆盖玉米全基因组的22对SSR引物,对180份玉米自交系的亲缘关系进行分子评价。结果显示:22对SSR引物共检测到129个等位基因,每一位点平均等位基因数(A)5.9,变幅2-13;平均基因多样性指数(He)和平均多态性信息含量(PIC)分别为0.583和0.528。基于模型的群体结构分析将所有材料分为5个类群,与国内自交系划分的杂种优势群体相一致。AMOVA分析表明,5个类群的遗传变异主要存在于群体内,群体间的遗传变异仅占总变异的3.38%,且达到极显著水平。不同群体间的遗传分化(Fst)在0.021-0.079之间。

    Abstract:

    22 SSRs covered the entire maize genome were selected to fingerprint a set of 180 maize inbred lines preserved in Institute of Crop Germplasm Resources, Shanxi Academy of Agricultural Sciences. Totally, 129 alleles were detected by these 22 SSRs, averaged 5.9 per locus, ranging from 2 to 13. The average gene diversity (He) was 0.583, and the overall PIC across all these SSRs was 0.528. Model-based clustering analysis indicated that all these 180 inbreds could be seperated into 5 distinct groups, agreed well with the heterotic groups of Chinese maize inbreds. Results from the F-statistics and the analysis of molecular variance (AMOVA) showed that the genetic differentiation coefficient among model-based groups varied from 0.021 to 0.079, and the overall genetic variation among these groups only accounted for 3.38%, the rest (96.62%) was observed existing within these groups.

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乔治军,刘龙龙,南晓洁,等.180份玉米自交系资源亲缘关系的分子评价[J].植物遗传资源学报,2011,12(2):211-215.

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