紫、红黄肉甘薯种质遗传多样性的ISSR分析
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国家科技条件平台项目“热带作物种质资源标准化整理、整合及共享试点”(No.2005DKA21000),中央级基本科研业务费“海南紫色甘薯种质资源评价(No.YJS-2008-S032)”。


Genetic Diversity Analysis through ISSR Markers for Purple, Red and Yellow Sweetpotato varieties
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1, National Science and Technology Platform; 2, Fundamental Scientific Research Operation Expenditure of Central Government.

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    摘要:

    采用ISSR分子标记,分析了21份紫肉、28份红黄肉甘薯种质遗传多样性。结果表明:17对引物共扩增出154条谱带,其中多态性谱带138条,占89.6%,平均每个引物扩增出8.12条多态性谱带,表现出丰富的多态性。聚类分析和主成分分析将49份甘薯种质聚为4大类,类型间遗传差异较大,将红黄薯单独聚为1类,说明紫薯和红黄薯分别具有明显不同的来源和系统演化关系。种质间的遗传相似系数变幅为0.58~0.93,其中,0.61~0.70之间的种质占51.4%,0.71~0.80之间的占44.0%。而邻近地域育种单位或同一育种单位的品种亲缘关系较近。文章对如何在育种中利用这些优异种质进行了讨论。

    Abstract:

    The genetic diversity of 21 purple and 28 red and yellow sweetpotato varieties has been evaluated by ISSR markers. There were 138 polymorphic alleles, 89.6% out of 154 amplified loci based on 17 selected ISSR primers with average 8.12 alleles each primer that shown a rich genetic diversity among the population. Clustering and principal component analysis showed that the all 49 sweetpotato clones had been divided into 4 groups with higher genetic distance among groups, and the red and yellow meat types have been ascribed into group Ⅱ. It indicated that there were different evolutionary relationship of purple, red and yellow sweetpotato germplasm. The coefficient of genetic conformability of all varieties were 0.58~0.93, 51.4% of germplasm were 0.61~0.70 and 44.0% of germplasm were 0.71-0.80. There was closer genetic relationship of the varieties come from the same or the nearby regions or breeding institute. It is discussed that how to use these varieties in sweetpotato breeding.

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黄洁,甘学德,苏明,等.紫、红黄肉甘薯种质遗传多样性的ISSR分析[J].植物遗传资源学报,2011,12(4):646-650.

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  • 收稿日期:2010-11-16
  • 最后修改日期:2011-02-08
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  • 在线发布日期: 2011-06-01
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